Journal: STAR Protocols

Article Title: A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

doi: 10.1016/j.xpro.2020.100224

Figure Lengend Snippet: Expected outcome of CellProfiler image analysis (A) Raw gray-scale image of a control well, recorded with the Opera Phenix high content screening system and merged using CombineTIFF software. Images are analyzed by open-source software CellProfiler. (B) Identification of the “Worm_4d” in a solvent control well (0.2% DMSO). Red number indicates worm area in pixel. (C) Identification of offspring (eggs + larvae) in a solvent control well (0.2% DMSO). Red numbers indicate the offspring count. (D) Example of a 4 μg/mL prochloraz treatment well containing a “Worm_4d_small”. Red number indicates worm area in pixel.

Article Snippet: Find the software tool (CombineTIFF.exe) and the associated code (CombineTIFF.m) in Mendeley Data (folder: CombineTIFF_Software_and_Code). a) Install v.9.2 of the MATLAB Runtime on a Windows-based operating system. b) Start “CombineTIFF.exe” and choose the Harmony export directory including raw images and the XML file. c) Optionally give images a suffix, select the channel(s) and choose to merge images without overlap. d) If single tiles are missing in a well, choose to skip those wells. e) In the input folder, CombineTIFF generates a new folder named “combinedTIFFs” in which the merged images are stored along with an info TXT file.

Techniques: High Content Screening, Software

Journal: STAR Protocols

Article Title: A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

doi: 10.1016/j.xpro.2020.100224

Figure Lengend Snippet:

Article Snippet: Find the software tool (CombineTIFF.exe) and the associated code (CombineTIFF.m) in Mendeley Data (folder: CombineTIFF_Software_and_Code). a) Install v.9.2 of the MATLAB Runtime on a Windows-based operating system. b) Start “CombineTIFF.exe” and choose the Harmony export directory including raw images and the XML file. c) Optionally give images a suffix, select the channel(s) and choose to merge images without overlap. d) If single tiles are missing in a well, choose to skip those wells. e) In the input folder, CombineTIFF generates a new folder named “combinedTIFFs” in which the merged images are stored along with an info TXT file.

Techniques: Recombinant, Software, Fluorescence, Microscopy, High Content Screening